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Annabella A. ADEWUNMI, Stephen O. FAPOHUNDA, Olumide AFOLABI, Abiodun JOSEPH. Occurrence of mycotoxigenic fungi in guinea corn and pearl millet marketed in South West Nigeria; Recent Advances in Food Science; 2021; 4(3): 341-357
Guinea corn (Sorghum bicolor) and Pearl millet (Pennisetum glaucum) marketed in South West Nigeria were investigated the for occurrence of mycotoxigenic fungi. A total of 32 pooled samples (17 sorghum and 15 millet) were taken from two major markets in each of the states in South-West Nigeria. Fungi were isolated from the grains on malt extract agar and identified by molecular method. Polymerase Chain Reaction (PCR) with Internally Transcribed Spacer (ITS) primers (ITS 1 and ITS 4) using DNA ladder (100bp - 3000bp) showed that the isolates were fungi while the DNA extraction with β-tubulin confirmed the presence of Aspergillus species and TEF1 gene confirmed Fusarium species. Gene sequencing of the amplicons identified predominantly Fusarium verticillioides, Fusarium coffeatum, Fusarium fujikuroi, Aspergillus niger and Aspergillus aculeatus. The nucleotide sequence data were submitted to the GenBank and were provided GenBank accession numbers. Some strains of these fungi have been known to be mycotoxigenic, with food safety implications.