Maria COJOCARU-TOMA, Robert ANCUCEANU, Mihaela DINU, Nicolae CIOBANU, Cristina CIOBANU, Tamara COTELEA, Mariana JIAN, Vitalie COBZAC, Viorel NACU, Maria-Mirabela TOMA; Viability and cytotoxicity evaluation on isolated hepatocytes of some species rich in polyphenolic compounds. Advanced Nano-Bio-Materials and Devices; 2020:4(2):580-585
The purpose of the study was evaluation of the cytotoxicity of the Agrimoniae herba and Cichorii herba extracts by counting the metabolic activity and the viability of the isolated hepatocytes from the laboratory animals, MTT technique (3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide). Hepatocytes were extracted from the rats of the Wistar line according to the two-stage hepatocyte isolation protocol. Further, cytotoxicity and cell viability of the hepatocytes were determined in vitro after their exposure to the extracts obtained from Agrimoniae herba and Cichorii herba in concentrations of 100, 200, 600 and 1000 mg/ml. The cell viability of hepatocytes treated with the extract from Agrimoniae herba in doses of 100 and 200 mg/ml are 92.78 ± 1.75 % and 79.23 ± 1.49 % and for Cichorii herba in doses of 100 mg/ml and 200 mg/ml constituted respectively 92.07 ± 1.44% and 76.07 ± 1.61 towards the control group; the dose of the 600 mg/ml presents 77.27 ± 2.38% for Agromoniae herba and 57.23 ± 1.38 % for Cichorii herba respectively. The lowest cell viability was attained at the concentration of 1000 mg/ ml, representing 41.6 ± 1.98 % for Cichorii herba and 49.98 ± 3.53 % for Agrimoniae herba, considered to manifest the highest cytotoxic effect. Doses of 100-200 mg/ml obtained from Agrimoniae herba and Cichorii herba demonstrate cell viability and don’t affect hepatocytes, respectively can be used in further studies.