The hyaline cartilage of the joint present high susceptibility to traumatic injury and degeneration and the ability of this tissue to regenerate is limited. Using a rabbit experimental model, our aim was to assess the Biomimetic scaffold capacity to recover the damaged cartilage by evaluation of Hounsfield Units (HU) on Computed Tomography scan. The biomimetic scaffold was represented by a monolit multi layered matrix. The superficial layer consists of type 1 equine collagen, the lowest layer consists of magnesium and hydroxyapatite and the middle layer consist of hydroxyapatite and collagen. The biomimetic scaffold promote bone and cartilage regeneration, by inducing selective differentiation of the body’s own bone marrow or synovial fluid derived progenitor cells into osteocytes, in the sub-chondral layer and chondrocytes in the cartilage layer. The HU of cartilage defect show higher values in the scaffold group compared with the control group at every time. Macroscopic and histological examination show regeneration of the chondral defect and formation of new hyaline-like tissue present only in the scaffold group.